Zoeller and Jacobsen won six skins at the two-day event and earned $320,000.
The 2007 winning duo of Jack Nicklaus and Tom Watson finished second after Nicklaus missed a 12-foot birdie putt at No. 18 in regulation. A make would have given the team its second straight Skins game victory.
The second-place finish was worth $270,000.
"It's a crazy game and things were just falling our way," Zoeller joked. "Guys were missing putts and we had an opportunity to make a couple to get us into the positive category and it just blossomed from there."
Gal Gadot cast as Wonder Woman for 'Batman vs. Superman'
Couple mistakenly served bag of cash at McDonald's drive-thru