On the game's critical play, quarterback Brock Berlin was intercepted by safety Gibril Wilson deep in Tennessee territory with less than five minutes left as the 17th-ranked Volunteers snapped Miami's 26-game at the Orange Bowl.
The last time Miami lost at home was Sept. 18, 1999, 27-23 to Penn State. The Hurricanes lost for the second straight week and effectively were eliminated from the national championship picture.
Tennessee (7-2) scored its only touchdown on a two-yard end-around run by wide receiver Derrick Tinsley late in the second quarter.
Miami's final chance ended when Sean Taylor fumbled a punt from Dustin Colquitt and Tennessee recovered before running out the clock.
Berlin completed 22-of-35 passes for 213 yards and was intercepted twice for the second straight week.