The video clip, posted to YouTube Thursday, features Obama checking his watch in the Oval Office when the vice president comes in and asks if the commander-in-chief is "ready to move," ABC News reported Friday.
The executives are then shown jogging through the hallways of the White House and outside of the building while workout music plays.
Obama and Biden are then shown stretching before returning inside for a glass of water.
"After a good workout, gotta drink up," Obama says. "Otherwise we're gonna get in trouble with (Biden's wife) Jill and Michelle."
The "Let's Move" campaign is intended to promote exercise and healthy living.