A majority of the players at the Peppermill Resort Spa Casino sported red-and-black "King Me" T-shirts as they made their pieces jump around their playing boards, the Reno Gazette-Journal reported.
"It was a pleasure to bestow the title of Most People Playing Checkers, or Draughts, Simultaneously to the PHP Agency in Reno," Guinness World Records adjudicator Philip Robertson said in a statement.
"As the international authority on the cataloguing and verification of every sort of record-setting accomplishment, it is fantastic to see the classic game of checkers represented in our collection of world records."
It was the first time the record had been attempted.
2014: The Year in Music [PHOTOS]