Pete and Penny, the penguins from SeaWorld, became the star attractions Wednesday, ABC News reported.
"When we reached cruising altitude, the captain allowed their handler to take them for a stroll up and down the aisle so that everyone could take a look at the cute, 1-foot-tall penguins and get some great photos and videos," passenger Jane Worthington Roth wrote on her YouTube page.
She uploaded three videos of the penguins on the plane.
The pilot and flight crew allowed trainers to let the penguins out of their kennels, to the delight of passengers.
"Oh my gosh! Look at him," one said.
"They're just so cute," said another.
"Pete and Penny are obviously enjoying their training to become flight attendants," Roth wrote. "You'll note that since they were sitting in first class, they are wearing their tuxedos!"
The two penguins were en route to New York for the premier of the upcoming Discovery Channel/BBC series "Frozen Planet."
"It's the first time I've ever seen everyone on a plane smiling at the same time!" Roth wrote.