Nike's flagship store on East 57th Street was forced to close Saturday, the New York Daily News reported.
"Our primary concern is the well-being of our consumers and sales associates," Nike's statement said. "We are taking all proper steps to eradicate the problem and we expect the store to reopen shortly."
The five-story Niketown, with big-name endorsers like Michael Jordan, Tiger Woods and Derek Jeter, sells wares ranging from expensive footwear to headbands to T-shirts.
Before hitting Niketown New York, the insects are known to have bitten into the Empire State Building, the Hollister store in SoHo, the Brooklyn district attorney's office, a Times Square movie theater and the Time Warner Center.
A study by the pest control company Terminix found New York had the nation's worst bedbug infestation.