The Columbus (Ohio) Dispatch reported Sunday that the program was aimed at combating rats in Columbus' downtown and university areas.
The newspaper said a 3 percent spending cut in the Columbus Public Health proposed budget for 2009 ensured that the $275,000 program won't be revived.
"In times of limited resources, we have to make tough decisions," Public Health spokesman Jose Rodriguez said. "This is not a required public-health function."
Federal officials say the city's rat population could rebound.
"If the right conditions are present for rodents to live, the population can rebound quickly," said Capt. Mike Herring, a senior environmental health officer at the U.S. Centers for Disease Control and Prevention.