The more than 300 "intimate" items were put up for sale by Jacqueline Kennedy Onassis' personal secretary and attendant, CNN reported.
The women had been storing the items for nearly 50 years.
The yellowed World War II Navy-issue shorts with "Jack Kennedy" sown in the band, opened at $700, CNN said. Within seconds, the item went to a telephone bidder for $5,000.
A campaign notebook used during the 1960 presidential campaign went for $22,500. The book contained 22 pages of notes, speech ideas and personal reminders.
A badly soiled autographed copy of Kennedy's 1961 inaugural address sold for $10,000 and a pair of JFK's pajama pants went for $2,000, CNN said.
A one-piece black bathing suit worn by Jackie Kennedy sold for $750.