The list of celebrities who have signed the bike also includes Nicolas Cage, John Travolta, Arnold Schwarzenegger, Sylvester Stallone, Cuba Gooding Jr., Salma Hayek, Pamela Anderson, Magic Johnson and Terry Bradshaw.
Visitors to eBay's Auction for America have been bidding on the bike, which Leno just bought in July, since last week.
It's black, with a custom black leather seat, has only about 200 miles on it, and is described as "in perfect condition."
On Oct. 10, Leno added a brand new limited edition 2001 Ford Harley-Davidson F-150 SuperCrew to the package. The SuperCrew is the second in a series of trucks jointly developed by Harley-Davidson and Ford.
After verifying that the top bidder has the funds to do the deal, Leno will announce the auction winner on Friday's "Tonight Show."
The list of signatures also includes Arsenio Hall, Leelee Sobieski, Tim Allen, Bill Maher, Mira Sorvino, Ed Harris, David Spade, Martina McBride, Megan Mullally, Michael Richards, Ed Kowalczyk, Drew Carey, Maria Bartiromo, Emeril Lagasse, Samuel L. Jackson, Jennifer Esposito, Michael Douglas, Jessica Alba, Ricky Martin, Jenna Elfman, Breckin Meyer, Billy Bob Thornton, Angelina Jolie, Doug Flutie, James Taylor, Russell Crowe, former Secretary of State Madeline Albright, Adam Sandler, Ellen DeGeneres, Martin Short, James Woods, Jennifer Garner, Ozzy Osbourne, Rodney Dangerfield, Heather Locklear, Matt LeBlanc, Matthew Perry, David Schwimmer, Jet Li and Jill Hennessy.
Images of both vehicles and celebrity signings are posted at nbcmv.com.