The sisters gave the camera their best "duck face" in a warm dusk light for the snapshot, posted Sunday on Jessica's account.
"Sunset with @missbananahammock / @ashleesimpsonofficial #SISTERS #NOFILTER," the fashion designer captioned the photo.
Jessica's post comes almost a week after she made headlines for sharing a photo of her 1-year-old son Ace playing with her best friend's CaCee Cobb's son, Rocco.
The last time the sisters posed together in an Instagram photo took place in January when Jessica shared a family photo of herself with Ashlee and her fiance, Evan Ross, just days after the two became engaged. The post was Jessica's second since opening her account on the image sharing site the day prior.