The 90-year-old former Price is Right host will be seen in Thursday's edition of the show.
He previously guest starred on the daytime drama this past April and in March 2002.
CBS said Barker will return to Spencer Publications to check on his pal Liam Cooper, played by Scott Clifton.
"But the friendly visit goes south when an argument between Barker and Wyatt Spencer, played by Darin Brooks, turns into a heated brawl. Infuriated by Wyatt's onslaught of insults toward animal lovers, Barker unleashes a flurry of punches, sending Wyatt rolling down a hill, where he lands face-down in a puddle of mud. The fight scene will once again proclaim Barker's ageless vigor, which was featured in the classic film Happy Gilmore in 1996," the network said in a synopsis.
"The Happy Gilmore fight scene was filmed almost 20 years ago and is still a favorite with audiences to this day. It was fun to revisit for The Bold and the Beautiful and to know that I can still throw a punch. It's proof that those karate lessons with Chuck Norris are still paying off," Barker said in a statement.
"At 90, Bob's still got it in 'spayeds' -- and neuters! I am so happy to have such a fine man and television legend on The Bold and the Beautiful. And doing his own stunts, no less," said executive producer and head writer Bradley Bell.