While signing autographs at The Normal Heart premiere May 19 in Beverly Hills, Calif., Ushkowitz broke the news to a videographer who asked if she was still dating Trevino, E! Online reported.
"No, we're not together anymore," she said.
"We're wonderful," she added when asked if the two were on good terms.
The two television actors began dating in 2011.
Celebrity Breakups and divorces of 2014 [PHOTOS]