The trip marks the 39-year-old host's first engagement outside New York since he took over for Jay Leno in February.
The Universal Orlando Resort will host the late night program from June 16 to June 19. A first look at the new Wizarding World of Harry Potter at Universal Studios is one highlight mentioned on the NBC website. Jennifer Lopez, Pitbull and Tracy Morgan are some of the guest stars scheduled to make appearances.
Tickets are free and become available May 21. Audience members must be at least 16 years old to attend.