The clip, which originally aired on Winfrey's show in November 2005, finds the now 60-year-old media mogul discussing the time she first learned about a talk show in Chicago that needed a new host.
"I stayed up all night long with an editor [and] sent my audition tape in," she says before airing the tape that she sent in 1983. "I was obsessed with getting this job."
In the clip, a 31-year-old Winfrey sports a beige and black blouse and cardigan combo and an afro hairstyle.
"Hi there," she says in the beginning of the tape. "My name is Oprah Winfrey. Oprah spelled O-P-R-A-H and if you notice, it's Harpo spelled backwards."
You can check out the talk show host explaining the origin of her name later in the tape, below.