Garber, the owner of Arbie's Team Transport of New Hampshire, was rushed to an undisclosed hospital Friday after suffering the heart attack and passed away soon after, TMZ reported.
Sources close to Garber told TMZ the reality TV star had from a heart condition for some time.
The website for "Shipping Wars" described Garber as a "master tradesman" and "know-it-all."
"We are deeply saddened by the loss of a member of the A&E family," said a statement from A&E posted to the show's Facebook page. "Our thoughts are with Roy's loved ones during this difficult time. He will be missed."
"Shipping Wars" just completed its fifth season.