The guys got together for a brief medley of their hits "Girlfriend," "Gone" and "Bye, Bye, Bye," which was part of 'NSYNC breakout star Justin Timberlake's 15-minute set of largely solo material.
Timberlake was then presented with the Video Vanguard Award.
Asked on the Florida radio program "Johnny's House" Monday if the reunion at the VMAs sparked plans for a concert tour or comeback album, Kirkpatrick said, "No."
"This was for Justin to get the Video Vanguard Award," he said.
"It was funny because all of us getting back together, we remembered what we love about each other and what we hate about each other," Kirkpatrick said. "It was just a bit of fun."
The popular 1990s vocal group last performed together at the Grammy Awards in 2003.