This marks the first time in four years Timberlake will take the Grammy stage. "The 20/20 Experience," Timberlake's first studio album in seven years, is set for release March 19.
The Grammy Awards show is to take place Feb. 10 at the Staples Center.
Additional performers, presenters and special segments will be announced soon, CBS said.
Previously announced performers for the ceremony include current nominees Dierks Bentley and Miranda Lambert, The Black Keys, fun., the Lumineers, Mumford & Sons, Rihanna, Taylor Swift, Carrie Underwood and Jack White. Elton John and Ed Sheeran are also to perform live together for the first time on the show.
LL Cool J is to serve as host of the awards presentation.