Greene's film credits include "Maverick," "Thunderheart" and "Dances with Wolves," for which he received a supporting actor Oscar nomination.
Set in the near future, "Defiance" introduces a completely transformed planet Earth, inhabited by the survivors of a universal war struggling to build a new society.
Greene will portray "Rafe McCawley, "a self-made success who owns and operates the largest mine in the territory," a synopsis said.
"However, Rafe's status as the richest and most powerful human in the town of Defiance comes with a price, as he struggles to navigate both familial and political alliances in this precariously assembled new world."
The cast also includes Grant Bowler, Julie Benz, Stephanie Leonidas, Tony Curran and Jaime Murray.