The clip featured Kimmel in various skin-tight or skimpy workout outfits, while hawking his Hottie Body fitness system, "Humpilates."
Eva Longoria and Sofia Vergara also appear in the video to offer testimonials about how the system changes their lives.
Lohan gets one of the clip's biggest laughs when Kimmel declares his workout system is so affordable it's a "steal," to which Lohan chirps, "allegedly."
Lohan, whose career has stalled in recent years due to her legal woes and battle with drug addiction, recently was charged with stealing a $2,500 necklace from a Los Angeles boutique. She has denied any wrongdoing in the incident.