The suit filed by Shane Stirling contends Barker had a major role in getting her bounced from the show shortly after she announced she was pregnant in 2006, TMZ.com said Sunday.
"The host of the game show had always been very kind and generous to Stirling until he learned that she was pregnant," the lawsuit said.
Stirling, who is seeking more than $25,000 in damages, alleged Barker insisted she not work while pregnant because of liability issues.
Stirling returned to work briefly in 2008 but was again let go because the show was "going in another direction." Barker had left the show by then, TMZ said.
Stirling argued she might have been able to keep her job had she returned to work earlier and been able get better acquainted with the new leadership.