Winfrey made the remarks about "Oprah: A Biography," Kitty Kelley's new book, Monday at The New York Women in Communications Matrix Awards, People.com said. It was the first time she commented publicly on the biography.
Winfrey had been on-hand at the event to present an award to her longtime friend and frequent collaborator Gayle King.
Winfrey told the crowd that last week "was a rough one" for King when a "so-called biography came out" because she "sometimes cares about my life more than I do."
"Gayle got herself worked out with all my new daddies coming out," Winfrey said. "This, too, shall pass."
Kelley claims in the book that Winfrey abused drugs and exaggerated the extent of the poverty she endured as a child, media reports have said. The biography also speculates about the identity of Winfrey's father, People.com said.