The actors play Charlie, Daniel and Desmond respectively. Sonya Walger is also expected to reprise her role of Penelope "Penny" Widmore.
They are to appear in Tuesday's episode of the supernatural series, the network said.
The iconic show is to end its six-season run in May.
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AUBURN HILLS, Mich., June 18 (UPI) --U.S. automaker Chrysler said Tuesday it would recall 2.7 million Jeeps after first snubbing regulators on the issue.