The wildly popular music artists are collaborators on the current hit song "Suit & Tie."
This will be Timberlake's only U.K. festival performance of the year.
"So this is your one chance to see him in a festival setting in 2013, performing songs from his new album, 'The 20/20 Experience,'" a message on his website said.
Jay-Z and Timberlake announced Friday they are planning a North American stadium concert tour together this summer.
Their "Legends of the Summer" show will stop in 12 cities, including New York, Boston, San Francisco, Los Angeles, Chicago and Toronto.