Weight Watchers is a sponsor of the event, which was organized in honor of O, the Oprah Magazine's 10th anniversary.
The walk is expected to raise money for 10 charities.
Hudson announced in April that for the past several months she has been successfully losing weight with the Weight Watchers program.
"Just like many Weight Watchers members out there, I've learned how to incorporate activity into my life and walking is something I love to do," Hudson, an Oscar winner and Weight Watchers spokeswoman, said in a statement. "I feel great and I am so excited to participate in the Live Your Best Life Walk and hope to inspire others to get moving this spring."
"We recognize that walking is one of Weight Watchers members' favorite ways of incorporating activity into their lives because it is easy and convenient. Walking in a group, especially, can be very motivating," said Theresa DiMasi, editor in chief of WeightWatchers.com. "The Live Your Best Life Walk is a natural partner for us -- it encourages people to get moving while supporting a worthy cause. It also coincides with Weight Watchers' own Walk-It Challenge, which encourages people to train for and walk a 5K."