UsMagazine.com said the original preview featured star Vince Vaughn speaking the line, "Ladies and gentlemen, electric cars ... are gay."
CNN news anchor Anderson Cooper and others have blasted the reference, saying the word "gay" is given a negative connotation in this instance, the report said.
"The teaser trailer for 'The Dilemma' was not intended to cause anyone discomfort. In light of growing claims that the introduction to the trailer is insensitive, it is being replaced. A full trailer, which has been in the works for some time, will post online later today," Universal said in a statement issued to UsMagazine.com Friday.
Tolerance, bullying and insensitivity toward gays have been major topics of discussion in the wake of the suicide last month of Rutgers University student Tyler Clementi, who jumped off the George Washington Bridge after his roommate allegedly streamed video online of him having sex with another man.