Focus Chief Executive Officer James Schamus made the announcement Thursday after the movie's premiere at the Sundance Film Festival in Utah.
"In the movie, two teenaged children conceived by artificial insemination get the notion to seek out their birth father and introduce him into the family life that their two mothers have built for them," Focus said in a news release.
Cholodenko directed the feature from an original screenplay she wrote with Stuart Blumberg.
The film stars Annette Bening, Julianne Moore, Mia Wasikowska, Josh Hutcherson and Mark Ruffalo.
"Lisa has made an extraordinary gem of a film that combines comedic surprise with poignant emotional truth. Not only is 'The Kids Are All Right' infectiously funny and touching, but it's also entirely accessible," Schamus said in a statement.
"'The Kids Are All Right' is all about family, and joining with Focus feels like a perfect marriage. I'm proud that the movie has now found such a special home," Cholodenko added.