The 41-year-old actress gave birth to a baby girl, and the couple named her Elizabella Dylan.
"Mommy and baby are doing wonderfully," Milano's rep said in a statement.
Milano and Bugliari are already parents to 3-year-old son Milo Thomas. The couple have been together since 2007, and celebrated their fifth wedding anniversary August 15.
"I loved waiting a long time to be a mom," the actress revealed in the August/September issue of Fit Pregnancy. "I'd had my ladies' lunches and my shopping days. When Milo finally came, I thought -- this is what I've waited for my entire life."
Milano is known for portraying Phoebe Halliwell on WB series Charmed, and currently stars as Savannah Davis on Mistresses. The ABC drama finished its second season September 1.