Prinze Jr. recently declared he had a "terrible" time with Sutherland on 24, and called the star "the most unprofessional dude in the world." Prinze Jr. portrayed Cole Ortiz on the show's eighth season, and said the experience was so bad he wanted to quit acting.
A representative for Sutherland tells ABC News the negative comments came as a surprise.
"Kiefer worked with Freddie Prinze, Jr. more than 5 years ago, and this is the first he has heard of Freddie's grievances," the rep said in response. "Kiefer enjoyed working with Freddie and wishes him the best."
The 47-year-old actor starred as Jack Bauer on eight seasons of 24, and reprised the character in limited series 24: Live Another Day. He will star with father Donald Sutherland and Demi Moore in Forsaken later this year, while Prinze Jr. has since appeared on Psych, Bones and Witches of East End.