Her character, Ran, won't be a player character, but a voice in the gamers' ear as they play.
"The first time I played EVE: Valkyrie I immediately knew I had to be a part of it," Sackhoff said in a statement. "Even with the roles I have played, it is the closest I have ever felt to being a real spaceship pilot. It is a truly transformative video game experience."
Sackhoff previously lent her voice to three other video games: Halo 3, Resistance 2 and Spider-man Edge of Time.
A release date for EVE: Valkyrie hasn't been announced.