Speculation that the pair are an item began after a source reportedly told Star Magazine that the two are dating and "having a great time together."
The actors met in 2011 on the set of Segel's sitcom How I Met Your Mother. Holmes appeared as a guest star to portray the character Naomi, aka "The Slutty Pumpkin," one of Ted's (Josh Radnor) love interests.
"The story is completely untrue," Holmes' representative Leslie Sloane tells Us Weekly. "[Holmes and Segel] have not spoken since she appeared on How I Met Your Mother."
Celebrity Couples of 2014 [PHOTOS]