The 1985 flick marked Tim Burton's first full-length debut as a director, and followed the character Pee-wee Herman on his adventurous nationwide search for his stolen bicycle.
Pee-wee professed in the film, "I wouldn't sell my bike for all the money in the world. Not for a hundred million, trillion, billion dollars!" The seller, who spoke to TMZ, feels differently.
The seller says the bike is currently in pieces, but that all the parts are there, including the bicycle bell and handlebar streamers. The person also says he has a letter from the studio to prove the bike's authenticity, and that he will throw in an autographed photo of Paul Reubens, the actor who played Pee-wee, as part of the deal.
The seller says he bought the bike for $10,000 in 2010, but hopes to get at least $15,000 for it on eBay. The current bid is $8,000.
See the bike, or place a bid, here. The auction ends on Thursday, March 6.