About 1,900 showed up to see the final performance of the musical on Broadway Saturday night, the New York Times reported.
The production cost $75 million, making it the most expensive musical in history. It was performed 1,268 times.
However, "Spider-Man," which is special effects-heavy, suffered a number of technical problems during its run -- during its first preview performance on Nov. 28, 2010, the show came to a complete stop five times for various reasons.
On Saturday, a stagehand had to run on stage during the performance to fully close a door to a "metamorphosis machine" to ensure the safety of an actor. The audience applauded the stagehand, the news report said.
Robert Cuccioli, who played the Green Goblin, thanked "the vast array of dedicated and talented people backstage" who had worked on the show as curtains closed.
Producers of the show are currently in talks with Las Vegas casino owner Steve Wynn about putting the musical into one of his properties and possibly adding more special effects.