Tsiskaridze has danced with the company for 20 years but will have to leave the theater June 30 after being notified "no new fixed-term employment contract will be prolonged," the theater's spokesman told RIA Novosti.
Tsiskaridze has been reprimanded in the past for giving media interviews without permission and publicly criticizing the theater.
RIA Novosti said the dancer's fans plan to picket in front of the Bolshoi Theatre next week to protest his ousting.
There was no word on where Tsiskaridze may perform next.
2014: The Year in Music [PHOTOS]