The first plaintiff withdrew his lawsuit from federal court this week after photos were published of Travolta in New York the day the incident was to have taken place in Los Angeles.
No reason was given for the second plaintiff's decision, the two accusers' attorney, Okorie Okorocha, told CNN Thursday.
Travolta's representatives offered no immediate comment on the development, but they have vehemently denied the allegations in the past.
The massage therapists have been identified publicly as only John Doe No. 1 and 2.
They claim the "Pulp Fiction" and "Grease" star groped and harassed them at the hotels where they work.
Travolta is married to actress Kelly Preston and is the father of her two children.