Natalie Mendoza, the actor who plays the character Arachne, was hit on the head by a rope during the show's first preview and sustained a concussion, the New York Post reported.
She is the third actor injured in the production in about six weeks. In the $65 million show, it is Mendoza's character that bites Peter Parker, turning him into "Spider-Man."
Mendoza was injured at the production's first preview Sunday.
"Natalie Mendoza suffered an injury earlier this week, and on doctors' orders will be out of the show for a brief period," Cohl said. "We look forward to welcoming her back to the production very soon."
In October an aerialist broke both wrists when he slammed onto the stage during a flying stunt, and in a different incident another actor broke his foot.
Mendoza's understudy, former Playboy cover model America Olivo, will take over the role of Arachne until she returns.