The game is to be shown on Fox Nov. 27.
The pop band's performance will officially kick off the 2008 Salvation Army Red Kettle Christmas campaign, which runs through Dec. 24.
Since the partnership between the charity and the football team began in 1997, the annual Red Kettle campaign has raised more than $1 billion, which the Salvation Army said has helped it serve 30 million people each year across the United States.
"I was born in Dallas, which makes performing with my brothers at the Cowboys' halftime on Thanksgiving an incredible honor," Nick Jonas said in a statement.
"This year, more than ever, the Salvation Army Red Kettle campaign will make a huge impact for families at Christmas and throughout the year. It's very important for us to give back," Joe Jonas added.
"There are no other artists more in touch with America's youth than Jonas Brothers," said Jerry Jones, the Cowboys' owner and general manager.