The event brought the cumulative total earned to more than $1 million since the show's inception in 2005.
The total was announced Tuesday by Brian Frons, president of Daytime Disney-ABC Television Group, and Tom Viola, executive director of Broadway Cares/Equity Fights AIDS.
Appearing at the event were stars from "All My Children," "One Life to Live," "General Hospital" and "The View."
The evening featured the ABC daytime stars singing and dancing in a variety of musical numbers, ABC said. Sierra Boggess and Sean Palmer from the hit Disney musical "The Little Mermaid" also made a special appearance.
Broadway Cares/Equity Fights AIDS is an industry-based, not-for-profit AIDS fundraising and grant-making organization.