The stars were among the slate of movies and television programs hailed at the big event Friday.
The AFI said in a release that Mirren closed the show with a tribute to director Robert Altman, who died in 2006. Mirren was in the cast of Altman's "Gossford Park" and was honored Friday for her performances as a pair of queens: Elizabeth II in the film "The Queen" and her namesake Elizabeth I in the television production "Elizabeth I."
Eastwood was among the directors honored while the surprise hit "Little Miss Sunshine" made the Top 10 films. Other movies on the list included "United 93," Happy Feet," "Borat" and Eastwood's "Letters From Iwo Jima."
Television honors went to newcomers "Friday Night Lights" and "Heroes" as well as stalwarts like "South Park," "West Wing" and "24."