The 55th NBA All-Star Game will be played Feb. 19 at the Toyota Center and broadcast live on TNT.
It will mark the final performance for the Grammy Award-winning trio, the NBA said in a news release.
Beyonce Knowles, Kelly Rowland and Michelle Williams announced their breakup last year.
Knowles is busy shooting "Dream Girls," Rowland is working on a solo release as well as UPN TV's "Girlfriends" and Williams is working on her solo album as well.
All three are Houston natives.