Rawls died Jan. 6 of lung cancer. The program listed his age as 72, although other records indicate he was 70, KABC-TV, Los Angeles reported.
"We're going to miss his love because it was real, it's pure," Wonder said. "We got you, Lou. We got you forever and always."
Jackson said Rawls was "authentic, an original, a source of light in dark places."
Rawls, who never went to college, raised more than $200 million for the United Negro College Fund with 27 years of telethons.
In his honor, UNCF President and CEO Michael Lomax announced a Lou Rawls lifetime achievement award to be presented to an artist during next year's telethon.