Blake, 71, was acquitted of murder charges in his wife's May 2001 shooting death outside a Studio City restaurant.
Bakley's four children filed suit against Blake in April 2002, but the civil case was put on hold until the criminal case concluded, KABC-TV, Los Angeles, reported Monday.
Attorneys for the heirs have not disclosed how much money they are seeking. They turned down a settlement offer of $250,000 from the former "Baretta" star.
The trial is expected to take three to six weeks.