Knowles, Kelly Rowland and Michelle Williams, who already have been in the studio recording, will reunite for a performance in September, with the concert taped to air Sept. 12 on ABC's "Pepsi's Play for a Billion" game show, E! Online reported Friday.
Pepsi's TV special, hosted by Damon Wayans, will award one Pepsi drinker up to $1 billion.
Destiny Child's sixth album will hit stores in November, followed by a tour.
The trio's 2001 "Survivor" sold more than 8 million copies and earned the group a Grammy.
During the hiatus, Knowles became an international star, with a successful solo record, film roles and fashion line; Rowland released her own Grammy-nominated solo debut and starred in the horror film "Freddy vs. Jason"; Williams released two solo gospel albums and starred in Broadway's "Aida."