In the film, the "X Files" star plays the straight brother of a drag queen who falls in love with a woman (Nia Vardalos) pretending to be a female impersonator.
"My history from 'Twin Peaks,' I'm the one usually wearing the dress," Duchovny joked with reporters in Los Angeles, "which is what I would have preferred, but they wouldn't let me and it's been 15 years since I did that and my (butt) isn't as good as it used to be."
Duchovny said he signed on to the film mainly because he enjoyed Vardalos' first outing, "My Big Fat Greek Wedding."
"I like Nia's voice as a writer and I like her persona as a performer and I loved the story of how 'My Big Fat Greek Wedding' became a huge monster whatever-it-was from nothing and I knew director Michael Lembeck just as a neighbor. ... That's why I wanted to do it," said the 43-year-old New York native.
"Connie and Carla," written by Vardalos and co-starring Toni Collette, opens Friday.