Gugino, who starred in TV's short-lived series "Karen Sisco," has been cast as Maggie in the Miller classic, Playbill Online reported Thursday.
The role of Maggie centers on a famous singer troubled by an addiction to sleeping pills and alcohol, which many believed is based on Miller's troubled marriage to Marilyn Monroe.
Mark Nelson, Jessica Hecht and Candy Buckley are also expected to be part of the ensemble cast, though no official announcement has been made.
The production is set to begin previews June 25 at the American Airlines Theatre, with the opening set for July 29.
"After the Fall" debuted on Broadway in 1964 with Jason Robards Jr. in the lead role and Barbara Loden as the singer, the role that won her a Tony for best featured actress. Hal Holbrook and Faye Dunaway also appeared in the play staged by Miller's collaborator, Elia Kazan.
2014: The Year in Fashion [PHOTOS]
Mindy Kaling mistaken for Malala Yousafzai