Timberlake will serve as both the host and musical guest on the show's Oct. 11 edition.
The singer is on a break from touring but will return to the road beginning Nov. 16 in Ghent, Belgium.
A DVD featuring music videos from Timberlake's "Justified" as well as live performance footage was released last week, Billboard said.
Spears will perform on the Oct. 18 episode, to be hosted by Halle Berry. She's expected to perform songs from her upcoming album "Get in the Zone," due to be released on Nov. 18. Her first single "More Than the Music," featuring Madonna, was scheduled to hit U.S. radio outlets Tuesday.